CORONAVIRUS qPCR PROBES AND PRIMERS

Custom Oligos

Specific Research Use Only (RUO) primers and probes for detection of severe acute respiratory syndrome coronavirus 2 - SARS-CoV-2 (previously known as “2019 novel coronavirus”) causing coronavirus disease (COVID-19).

Primer and Probe Sets from National Institute For Viral Disease Control and Prevention / China

(see reference)

Name Description Oligonucleotide Sequence (5’>3’)
2019-nCoV_ORF1ab-F 2019-nCoV_ORF1ab Forward Primer 5’-CCC TGT GGG TTT TAC ACT TAA-3’
2019-nCoV_ORF1ab-R 2019-nCoV_ORF1ab Reverse Primer 5’-ACG ATT GTG CAT CAG CTGA-3’
2019-nCoV_ORF1ab-P 2019-nCoV_ORF1ab Probe 5’-FAM-CCG TCT GCG GTA TGT GGA AAG GTT ATG G-BHQ1-3’
2019-nCoV_N-F 2019-nCoV_N Forward Primer 5’-GGG GAA CTT CTC CTG CTA GAA T-3’
2019-nCoV_N-R 2019-nCoV_N Reverse Primer 5’-CAG ACA TTT TGC TCT CAA GCT G-3’
2019-nCoV_N-P 2019-nCoV_N Probe 5’-FAM-TTG CTG CTG CTT GAC AGA TT-BHQ1-3’


Primer and Probe Sets from Centers for Disease Control and Prevention / CDC, USA

(see reference)

Name Description Oligonucleotide Sequence (5’>3’)
2019-nCoV_N1-F 2019-nCoV_N1 Forward Primer 5’-GAC CCC AAA ATC AGC GAA AT-3’
2019-nCoV_N1-R 2019-nCoV_N1 Reverse Primer 5’-TCT GGT TAC TGC CAG TTG AAT CTG-3’
2019-nCoV_N1-P 2019-nCoV_N1 Probe 5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3’
2019-nCoV_N2-F 2019-nCoV_N2 Forward Primer 5’-TTA CAA ACA TTG GCC GCA AA-3’
2019-nCoV_N2-R 2019-nCoV_N2 Reverse Primer 5’-GCG CGA CAT TCC GAA GAA-3’
2019-nCoV_N2-P 2019-nCoV_N2 Probe 5’-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3’
2019-nCoV_N3-F 2019-nCoV_N3 Forward Primer 5’-GGG AGC CTT GAA TAC ACC AAA A-3’
2019-nCoV_N3-R 2019-nCoV_N3 Reverse Primer 5’-TGT AGC ACG ATT GCA GCA TTG-3’
2019-nCoV_N3-P 2019-nCoV_N3 Probe 5’-FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1-3’
RP-F RNAse P Forward Primer 5’-AGA TTT GGA CCT GCG AGC G-3’
RP-R RNAse P Reverse Primer 5’-GAG CGG CTG TCT CCA CAA GT-3’
RP-P RNAse P Probe 5’-FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ-1-3’

RECOMMENDATION:

To achieve the highest accuracy of virus testing, it is necessary to consider that:

  1. The CDC primers for SARS-CoV-2 have a lot of background (see reference)
  2. The SARS-CoV-2 has almost 90% similarity to other SARS-like coronaviruses, which can cause confusion in detecting the right virus (see reference)
For the most accurate qPCR results:
1) We will synthesize custom primers and probes of your choice
2) We will offer design of the most specific primers and TaqMan probes


AMOUNTS:

For 500 rxn kit, we offer 10 nmol of reverse and forward primer and 2.5 nmol of TaqMan probe.


PRICE:

MDX SARS-CoV-2 RUO Kit, 100 rxn - €49,00

MDX SARS-CoV-2 RUO Kit, 500 rxn - €98,00

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Coronavirus qPCR probes and primers

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