rTEST COVID-19 qPCR B.1.1.7 kit


Main features

  • Duplex configuration: Innovative dual TaqMan® probes for consensus (C95) and B.1.1.7 Spike (S) genes are conjugated to FAM and the TaqMan® probe for RNase P is conjugated to Cy5 or Yakima Yellow® 
  • At least one-month room temperature stability 
  • Innovative dual TaqMan® probe
  • Improved sensitivity of C95 and B.1.1.7 assays multiplexed with RNase P assay as an internal control 
  • SARS-CoV-2 B.1.1.7 full genomic RNA spiked with human RNA used as a positive control 
  • 100% clinical accuracy and specificity for both the consensus SARS-CoV-2 and B.1.1.7 variant 
  • 100% analytical sensitivity with 2 copies/rxn for consensus SARS-CoV-2 S gene and 10 RNA copies/rxn for B.1.1.7 variant
  • Validated on real lineage B.1.1.7 clinical samples confirmed by sequencing
  • No cold chain shipping needed 



Product description

The rTEST COVID-19 B.1.1.7 qPCR kit is a qualitative in vitro confirmatory test designed for specific detection of the B.1.1.7 variant of SARS-CoV-2 first described by the United Kingdom. This coronavirus lineage is associated with increased transmissibility. The kit contains two sets of primers and hydrolysis probes (TaqMan®) targeting either the consensus (C95) sequence of the SARS-CoV-2 Spike (S) gene or select mutations in the S gene of the B.1.1.7 variant. Both sets allow the detection of the human RNase P gene. The TaqMan® probes for the S gene of both the consensus SARS-CoV-2 and B.1.1.7 variant are conjugated to FAM, while the TaqMan® probe for RNase P is conjugated to Cy5 or Yakima Yellow®. This enables multiplexed detection of either the consensus SARS-CoV-2 S gene or B.1.1.7 variant, and human RNase P, which serves as an internal control to validate proper sample collection, RNA extraction, and performance of the test. The 5X One-step Probe CoV Mix (ROX) reagent from Solis BioDyne includes a ROX passive reference dye with a unique molecular structure allowing our RT-qPCR test to be compatible with all ROX-dependent and ROX-independent real-time PCR cyclers. 

One package of the kit is sufficient for 400 testing reactions and is intended to determine the B.1.1.7 variant in patient samples that tested SARS-CoV-2 positive in the primary test. It is recommended to run two parallel reactions to detect the presence of consensus SARS-CoV-2 S gene in one reaction and the B.1.1.7 variant in another one.

Registration code: P2058A

MultiplexDX, s.r.o.
IČO: 50111965
Ilkovičova 8, 841 04 Bratislava, Slovenská republika
+421 2 902 68 310

© 2020 MultiplexDX, s.r.o. All rights reserved.
rTest COVID-19 is a trademark of MultiplexDX, Ltd.
All other product names and trademarks are the property of their respective owners


rTest COVID-19 qPCR B.1.1.7 Kit

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