rTEST COVID-19 qPCR Multiplex Kit


Main features

  • At least one-month room temperature stability
  • Improved sensitivity of RdRP and E assays multiplexed  with RNase P assay as an internal control
  • Innovative dual TaqMan® probes
  • SARS-CoV-2 full genomic RNA spiked with human RNA used as a positive control
  • 100% clinical accuracy and specificity for SARS-CoV-2 
  • Great compatibility
  • 100% analytical sensitivity with 2 RNA copies/rxn
  • Validated on real clinical samples
  • No cold chain shipping needed 



The rTEST COVID-19 qPCR kit is an innovative, novel
and proprietary version of the WHO-recommended Charité, Berlin protocol. 

The kit contains two sets of primers and TaqMan® probes targeting the SARS-CoV-2 specific E and RdRP genes multiplexed with human RNase P. E and RdRP genes are detected in FAM channel and RNase P in HEX, JOE or VIC channel. This enables multiplexed detection of SARS-CoV-2 (confirmation of positive COVID-19) and human RNA, which can be used for secondary confirmation of negative samples to eliminate false-negative results as well as validation of sample collection and RNA extraction. For E and RdRP genes, we developed dual TaqMan® probes to increase the sensitivity and specificity of our 1-Step RT-qPCR method. 

The 5X One-step Probe CoV Mix (ROX) reagent from
Solis BioDyne includes ROX passive reference dye with a unique molecular structure allowing our RT-qPCR method to be compatible with all ROX-dependent and ROX-independent real-time PCR cyclers. 

A single kit is sufficient for 400 test reactions. It offers flexibility
in testing by permitting either single gene screening or precision dual gene diagnostics. E or RdRP gene (based on the end-user preference) can be exclusively used for 400 screening tests. This option is recommended when many symptomatic patients have to be screened at once. The precision dual gene option is based on running 200 screening assays first (e.g., E gene) followed by 200 confirmation assays to confirm positive screening results (e.g., RdRP gene) or eliminate false-positive results. For the precision dual gene diagnostics, if necessary, two testing reactions should be performed per one patient. 

For more info see User Information

Registration code: P1430A

MultiplexDX, s.r.o.
IČO: 50111965
Ilkovičova 8, 841 04 Bratislava, Slovenská republika
+421 2 902 68 310

© 2020 MultiplexDX, s. r.o. All rights reserved.
rTest COVID-19 is a trademark of MultiplexDX, Ltd.
All other product names and trademarks are the property of their respective owners


rTest COVID-19 qPCR Multiplex Kit

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